-LR3-IGF-1 Reconstitution Instructions:

To note:Whether your IGF is in lyophilized/powder form or in solution

STORE IT IN THE REFRIGERATOR NOT THE FREEZER!

DO NOT USE BA!USE ONLY ACETIC ACID!!!



1mg of lyophilized LR3-IGF-1





It's easier to use 2ml acetic acid because it is easier to measure later

whenyou draw it into a syringe.



After removing the vacuum SLOWLY add AA to the vial. try not to let the

stream of aa hit the powder directly, try to let it run down the glass

into the powder. when the aa is in gently swirl the vial until all is

disolved. do not shake.



a 1ml syringe is marked like this

llll10llll20llll30llll40llll50llll60...etc a 1/2 ml syringe is like this

llll5llll10llll15llll20llll25llll30



on a 1ml each line represents 2 units 2,4,6,8,10

on a 1/2 ml each line is 1 unit 1,2,3,4,5,6,7,8,9,10. this makes it

easier to measure.



so if you use 1ml of aa, on a 1ml pin, each line is 20mcg. on a 1/2ml pin

each line is 10mcg



if you use 2ml of aa, on a 1ml pin each line is 10mcg, on a 1/2 ml each

line is 5mcg...the 5line is 25mcg, 10 line is 50mcg...etc



so you see that the easiest way to measure is using 2ml and a 1/2ml pin



your igf is stored in aa solution, nacl is added to dilute at the time of

research. draw twice as much nacl as igf. so if you draw igf to the ten

line, draw nacl to the 30 line and you are ready to go.